O'Connell, N. McClure, S. Morphology was assessed using Tygerberg's strict criteria and viability was assessed by eosin Y. Sperm motility was measured using computer-assisted semen analysis CASA.

The aim of this study is to evaluate which cryopreservation protocol, freezing before or after swim-up, optimizes cryopreservation outcomes in terms of motile sperm count, motility, morphology, and viability, and also to establish whether sperm viability could be assessed based on sperm motility. Fifty-three fresh and 53 swim-up prepared samples were considered for the first experiment. In parallel, total motility evaluation by CASA system computer-assisted sperm analyzer and hypoosmotic swelling test HOS-test was performed in each sample to compare the viability results of both methods. In the second experiment, 21 normozoospermic semen samples and 20 semen samples from male factor patients were included. After fresh ejaculate evaluation, the semen sample of each patient was divided into two aliquots, one of them was frozen before swim-up and the other was frozen after swim-up. Motility, sperm count, morphology, and viability were evaluated after thawing.

Semen cryopreservation commonly called sperm banking or sperm freezing is a procedure to preserve sperm cells. Semen can be used successfully indefinitely after cryopreservation. For human sperm, the longest reported successful storage is 24 years. It is also often used by transgender women prior to medically transitioning in ways that affect fertility, such as hormone replacement therapy and orchiectomies. Often sucrose or other di- , trisaccharides are added to glycerol solution.

What it's like to freeze and bank sperm from start to finish, according to experts. If you're looking to start a family at some point, you and your partner may be looking into freezing sperm. But getting the facts about what it's like to freeze your sperm and sperm banking can be elusive. We asked two fertility experts about some of the most frequently asked questions about what it is like to freeze your sperm and use a sperm bank. The sample can be collected in the privacy of their home and shipped overnight to the laboratory for testing and freezing," says Dr.